CIP Code:   40.0502  
 
	METROPOLITAN STATE COLLEGE OF DENVER 
	Office of Academic Affairs 
 
	REGULAR COURSE SYLLABUS 
 
SCHOOL:   Letters, Arts & Sciences 
DEPARTMENT:  Chemistry  
SEMESTER(S) OFFERED:   Spring  
PREFIX & COURSE NUMBER:   CHE 411 
COURSE TITLE:   Instrumental Analysis Laboratory 
CREDIT HOURS:   2   ( 0 + 6 ) 
CONTACT HOURS:  Lecture  0 	Lab  90 	Internship   0 	Practicum   0  
RESTRICTIONS (VARIABLE TOPICS COURSES):   None 
PREREQUISITE(S):   CHE 300, CHE 301, CHE 310, CHE 311; CHE 319 or 326 
COREQUISITE(S):   CHE 410 
CATALOG COURSE DESCRIPTION: 
 
A study of instrumental methods, employing electromagnetic radiation, magnetic resonance,  
mass spectrometry, chromatography, and electrochemistry. 
 
REQUIRED READING MATERIALS  (Title, Author, Publisher, Copyright Date): 
 
No text book will be required for this laboratory.  Information will be obtained from current  
Chemistry Journals and from handouts. 
 
 
SPECIFIC (MEASURABLE) STUDENT BEHAVIORAL LEARNING OBJECTIVES: 
 
Upon completion of this course the student should be able to: 
 
1.	Describe in words and diagram the schematic of each instrument listed in the course  
outline. 
2.	Describe the theory of operation of each instrument listed in the course outline. 
3.	Describe the application and limitations of each instrument listed in the course outline. 
4.	Determine the structure of common organic compounds from the results obtained from  
these instruments. 
5.	Perform quantitative analysis on instruments whenever feasible. 
6.	Interpret simple mass spectrum, IR and NMR spectrum. 
7.	Perform chromatographic separation and clean ups. 
8.	Use several types of detectors for HPLC and GC. 
 
OUTLINE OF COURSE CONTENT (Major Topics and Subtopics): 
 
A.	Gas Chromatograph 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
B.	Infrared Spectrophotometer 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
C.	Mass Spectometer 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
D.	Ultraviolet-Visible Spectrophotometer 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
 
 
E.	HPLC 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
F.	Atomic Absorption 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
G.	Spectrofluorometer 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
H.	NMR 
	a.	instrument operation 
	b.	hands-on experience 
		-	sample preparation 
		-	instrumental analysis 
		-	interpretation of results 
 
EVALUATION OF STUDENT PERFORMANCE: 
 
Students will write formal reports on each analysis performed.  These reports will be graded  
on content and accuracy of the analysis.  A written final examination will be given.  The final  
grade will be determined by averaging the grades from the reports and the final examination. 
 
Revised 9/94:	Academic Affairs-Curriculum-Regular Course Syllabus 
					(s:\wpform\acadaff\currculm\regsyl.wp)