CIP Code:   40.0502 
HEGIS Code:    1909 
	METROPOLITAN STATE COLLEGE OF DENVER
	Office of Academic Affairs

	REGULAR COURSE SYLLABUS

SCHOOL:   Letters, Arts & Sciences
DEPARTMENT:  Chemistry 
SEMESTER(S) OFFERED:   Fall, Spring 
PREFIX & COURSE NUMBER:   CHE 432 
COURSE TITLE:   Biochemistry II
CREDIT HOURS:   3   ( 3 + 0 )
CONTACT HOURS:  Lecture  45 	Lab  0 	Internship   0 	Practicum   0 
RESTRICTIONS (VARIABLE TOPICS COURSES):   None
PREREQUISITE(S):   CHE 431 or permission of instructor.
COREQUISITE(S):   None
CATALOG COURSE DESCRIPTION:

Continuation of Biochemistry I (CHE 431).  Course theme is regulation, and emphasis is 
current biochemical research results.  Topics covered include enzyme catalysis, biosynthesis, 
membrane biochemistry, genetic engineering, molecular basis of cancer, neurochemistry, 
biochemical detoxification, molecular basis of inherited diseases, free radical theory of aging, 
and selected topics in human nutrition.

REQUIRED READING MATERIALS  (Title, Author, Publisher, Copyright Date):

Biochemistry, Mathews and van Holde, Benjamin/Cummings, 1990.


SPECIFIC (MEASURABLE) STUDENT BEHAVIORAL LEARNING OBJECTIVES:

Upon completion of this course the student should be able to:

1.	Recognize and identify the major classes of biomolecules and discuss their biochemical 
and nutritional significance:  lipids, amino acids, nucleotides, saccharides and metabolites 
of intermediary metabolism.
2.	Solve bioenergetic equations relating to energy transformations in the mitochondrion, 
muscle cell and nerve cell.
3.	Discuss regulation of biochemical systems on the following levels:
	A.	Feedback inhibition and activation of individual branch point enzymes.
	B.	Cascade regulatory control via protein phosphorylation.
	C.	Genetic regulation of enzyme production.
4.	Discuss the biochemical basis for recent advances in molecular biology with respect to 
biotechnology and cancer:
	A.	Gel electrophoresis
	B.	Restriction enzymes
	C.	transposition of genetic elements

OUTLINE OF COURSE CONTENT (Major Topics and Subtopics):

I.	Catalysis
	A.	Enzyme, Michaelis-Menten Kinetics
	B.	Enzyme, Allosteric Kinetics
II.	Biosynthesis
	A.	Lipids
	B.	Amino Acids
	C.	Nucleotides
III.	Topics in Molecular Biology
	A.	DNA sequencing
	B.	Mutagenesis
	C.	Molecular basis of cancer
	D.	Transposition and genetic recombination
	E.	Gene regulation
IV.	Molecular Basis of Inherited Disease
	A.	Lesch-Nyhan Syndrome
	B.	Gylcogen Storage disease
	C.	Phenylketonuria
	D.	Galactosuria, galactosemia
	E.	Lactose intolerance
V.	Neurochemistry
	A.	Action Potentials
	B.	Na+, K+ -ATPase; Bioenergetics of ion transport
	C.	Synapse and neurotransmission
	D.	Mechanisms of phychoactive drugs
VI.	Naturally Occurring Toxins
	A.	Oxygen
	B.	Plant toxins (laetrile, cycasin)
	C.	Microbial toxins (cholera, diphtheria)
	D.	Antibiotics and antiviral agents (P-450)
VII Biochemical Detoxification
	A.	Alcohol metabolism and alcoholism
	B.	Liver function in detoxification(cytochrome)
VIII Topics in Human Nutrition
	A.	Serotonin and diet
	B.	Physiology of Starvation
	C.	Sources of muscular energy
	D.	Sugar and diet
	E.	Cholesterol, lipoproteins and heart

EVALUATION OF STUDENT PERFORMANCE:

Critiques of current research papers;  Term paper of 8-12 pages which reviews a current 
research area in biochemistry.  Oral presentation of term paper and facilitation of class 
discussion.  Final exam.

Copies retained by Dean and Department Chair

Revised 9/94:	Academic Affairs-Curriculum-Regular Course Syllabus
					(s:\wpform\acadaff\currculm\regsyl.wp)